Publication Information

{'id': 'https://openalex.org/W2359248049', 'doi': None, 'title': 'DNA sequencing using 230 kb DNA of virus as template', 'display_name': 'DNA sequencing using 230 kb DNA of virus as template', 'publication_year': 2004, 'publication_date': '2004-01-01', 'ids': {'openalex': 'https://openalex.org/W2359248049', 'mag': '2359248049'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTOTAL-JNDX200402007.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764667412', 'display_name': 'Journal of Jinan University', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4320800752', 'host_organization_name': 'Jinan University', 'host_organization_lineage': ['https://openalex.org/P4320800752'], 'host_organization_lineage_names': ['Jinan University'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5100931066', 'display_name': 'Chunfang Xie', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': True, 'raw_author_name': 'Xie Chun-fang', 'raw_affiliation_strings': ['Dept'], 'affiliations': [{'raw_affiliation_string': 'Dept', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 0, 'institutions_distinct_count': 0, 'corresponding_author_ids': ['https://openalex.org/A5100931066'], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 61}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10981', 'display_name': 'Cytomegalovirus and herpesvirus research', 'score': 0.9791, 'subfield': {'id': 'https://openalex.org/subfields/2713', 'display_name': 'Epidemiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10981', 'display_name': 'Cytomegalovirus and herpesvirus research', 'score': 0.9791, 'subfield': {'id': 'https://openalex.org/subfields/2713', 'display_name': 'Epidemiology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/dna-nanoball-sequencing', 'display_name': 'DNA nanoball sequencing', 'score': 0.8298814}, {'id': 'https://openalex.org/keywords/sequencing-by-ligation', 'display_name': 'Sequencing by ligation', 'score': 0.7205866}, {'id': 'https://openalex.org/keywords/in-vitro-recombination', 'display_name': 'In vitro recombination', 'score': 0.5866174}, {'id': 'https://openalex.org/keywords/primer', 'display_name': 'Primer (cosmetics)', 'score': 0.53833055}, {'id': 'https://openalex.org/keywords/multiple-displacement-amplification', 'display_name': 'Multiple displacement amplification', 'score': 0.46331358}, {'id': 'https://openalex.org/keywords/sequencing-by-hybridization', 'display_name': 'Sequencing by hybridization', 'score': 0.4423563}, {'id': 'https://openalex.org/keywords/dna-clamp', 'display_name': 'DNA clamp', 'score': 0.42259446}], 'concepts': [{'id': 'https://openalex.org/C54887055', 'wikidata': 'https://www.wikidata.org/wiki/Q5205758', 'display_name': 'DNA nanoball sequencing', 'level': 5, 'score': 0.8298814}, {'id': 'https://openalex.org/C52182516', 'wikidata': 'https://www.wikidata.org/wiki/Q17144128', 'display_name': 'Sequencing by ligation', 'level': 5, 'score': 0.7205866}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.7045492}, {'id': 'https://openalex.org/C51679486', 'wikidata': 'https://www.wikidata.org/wiki/Q380546', 'display_name': 'DNA sequencing', 'level': 3, 'score': 0.6287202}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.6022217}, {'id': 'https://openalex.org/C203284983', 'wikidata': 'https://www.wikidata.org/wiki/Q17087215', 'display_name': 'In vitro recombination', 'level': 5, 'score': 0.5866174}, {'id': 'https://openalex.org/C129312508', 'wikidata': 'https://www.wikidata.org/wiki/Q133686', 'display_name': 'Oligonucleotide', 'level': 3, 'score': 0.5573501}, {'id': 'https://openalex.org/C141231307', 'wikidata': 'https://www.wikidata.org/wiki/Q7020', 'display_name': 'Genome', 'level': 3, 'score': 0.5449927}, {'id': 'https://openalex.org/C2777563447', 'wikidata': 'https://www.wikidata.org/wiki/Q7243397', 'display_name': 'Primer (cosmetics)', 'level': 2, 'score': 0.53833055}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.526085}, {'id': 'https://openalex.org/C156385826', 'wikidata': 'https://www.wikidata.org/wiki/Q1341059', 'display_name': 'Multiple displacement amplification', 'level': 5, 'score': 0.46331358}, {'id': 'https://openalex.org/C51064596', 'wikidata': 'https://www.wikidata.org/wiki/Q7452482', 'display_name': 'Sequencing by hybridization', 'level': 5, 'score': 0.4423563}, {'id': 'https://openalex.org/C31254050', 'wikidata': 'https://www.wikidata.org/wiki/Q22328799', 'display_name': 'DNA clamp', 'level': 5, 'score': 0.42259446}, {'id': 'https://openalex.org/C13575015', 'wikidata': 'https://www.wikidata.org/wiki/Q1562492', 'display_name': 'Genomic library', 'level': 4, 'score': 0.33070272}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.31415597}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.3050316}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.29190096}, {'id': 'https://openalex.org/C78063203', 'wikidata': 'https://www.wikidata.org/wiki/Q3062596', 'display_name': 'DNA extraction', 'level': 4, 'score': 0.25065622}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.24163684}, {'id': 'https://openalex.org/C19924922', 'wikidata': 'https://www.wikidata.org/wiki/Q408351', 'display_name': 'Molecular cloning', 'level': 4, 'score': 0.23343372}, {'id': 'https://openalex.org/C187882448', 'wikidata': 'https://www.wikidata.org/wiki/Q283478', 'display_name': 'Complementary DNA', 'level': 3, 'score': 0.19105443}, {'id': 'https://openalex.org/C70712619', 'wikidata': 'https://www.wikidata.org/wiki/Q908889', 'display_name': 'DNA sequencer', 'level': 4, 'score': 0.18071723}, {'id': 'https://openalex.org/C156719811', 'wikidata': 'https://www.wikidata.org/wiki/Q28774', 'display_name': 'Reverse transcriptase', 'level': 4, 'score': 0.10373217}, {'id': 'https://openalex.org/C3017666073', 'wikidata': 'https://www.wikidata.org/wiki/Q1764062', 'display_name': 'Base sequence', 'level': 3, 'score': 0.08785534}, {'id': 'https://openalex.org/C178790620', 'wikidata': 'https://www.wikidata.org/wiki/Q11351', 'display_name': 'Organic chemistry', 'level': 1, 'score': 0.0}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTOTAL-JNDX200402007.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764667412', 'display_name': 'Journal of Jinan University', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4320800752', 'host_organization_name': 'Jinan University', 'host_organization_lineage': ['https://openalex.org/P4320800752'], 'host_organization_lineage_names': ['Jinan University'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W3179976156', 'https://openalex.org/W3113449875', 'https://openalex.org/W3067383415', 'https://openalex.org/W3020932253', 'https://openalex.org/W2865313833', 'https://openalex.org/W2467818627', 'https://openalex.org/W2092030043', 'https://openalex.org/W2090791734', 'https://openalex.org/W2078941206', 'https://openalex.org/W2076321102', 'https://openalex.org/W2069820575', 'https://openalex.org/W2039962084', 'https://openalex.org/W2025570276', 'https://openalex.org/W2021243296', 'https://openalex.org/W2018374847', 'https://openalex.org/W1992014970', 'https://openalex.org/W1991405561', 'https://openalex.org/W1968612093', 'https://openalex.org/W1848030807', 'https://openalex.org/W1484191503'], 'abstract_inverted_index': {'Aim:': [0], 'To': [1], 'verify': [2], 'that': [3, 129], 'the': [4, 17, 26, 54, 107, 111, 130], 'Tn3-gpt': [5, 50, 131], 'was': [6, 65, 71, 97, 127, 132], 'inserted': [7, 133], 'in': [8, 134, 152], 'specific': [9, 135], 'site': [10, 136], 'of': [11, 19, 28, 35, 62, 137], 'MCMV': [12, 36, 48, 138], '(mouse': [13], 'cytomegalovirus)genome': [14], 'and': [15, 58, 85, 115, 122], 'predigest': [16], 'method': [18, 27, 78], 'large': [20, 146], 'fragment': [21, 148, 151], 'DNA': [22, 29, 34, 61, 69, 75, 82, 96, 147, 153, 160], 'sequencing,': [23], 'we': [24], 'established': [25], 'sequencing': [30, 70, 77], 'using': [31, 79], '230': [32, 59, 80, 93, 158], 'kb': [33, 60, 81, 94, 159], 'as': [37, 83, 88, 103, 161], 'template': [38, 84, 104, 162], 'directly.': [39, 105, 163], 'Methods:': [40], 'The': [41, 91], 'NIH': [42], '3T3': [43], 'cells': [44], 'were': [45, 56], 'infected': [46], 'with': [47], 'containing': [49], 'insertion.': [51], 'After': [52], 'incubation': [53], 'virions': [55], 'prepared': [57], 'virus': [63], 'genome': [64, 95], 'purified': [66], 'from': [67], 'media.': [68], 'carried': [72], 'out': [73], 'by': [74], 'cycle': [76], 'synthetic': [86], 'oligonucleotide': [87], 'primer.': [89], 'Results:': [90], 'pure': [92], 'obtained,': [98], 'which': [99], 'can': [100], 'be': [101], 'used': [102], 'In': [106], 'obtained': [108], 'gel': [109], 'picture': [110], 'bands': [112, 119], 'are': [113, 120], 'clear': [114], 'intensity,': [116], 'spaces': [117], 'between': [118], 'even': [121], 'easy': [123], 'to': [124, 144, 149], 'read.': [125], 'It': [126], 'verified': [128], 'genome.': [139], 'Conclusion:It': [140], 'is': [141], 'not': [142], 'necessary': [143], 'cut': [145], 'small': [150], 'sequencing.': [154], 'We': [155], 'could': [156], 'use': [157]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2359248049', 'counts_by_year': [], 'updated_date': '2024-12-11T01:49:00.489889', 'created_date': '2016-06-24'}