Title: The Role of Oxidative Stress in Hypoxia/Reoxygenation Induced Injury in Vascular Endothelial Cells
Abstract: Objective To detect the mechanisms by which hypoxia/reoxygenation(H/R) injuries coronary arteries endothelial cell(CAECs) and the effect of an anti-oxidative agent, pyrrolidine dithiocarbamate(PDTC). Methods Porcine CAECs were cultured and divided into three groups. Using untreated CAECs as negative control, the cells received H/R injury with or without PDTC pre-treatment. The levels of glutathione(GSH) and malonaldehyde(MDA) in the supernatant were quantitatively assayed. The expression of intercellular adhesion molecule-1(ICAM-1) in CAECs was tested by immunocytochemistry. Results There were no obvious changes of GSH, MDA and ICAM-1 in the control group (P0.05). H/R injury significantly decreased the GSH level (P0.05), associated with increase in MDA level and ICAM-1 expression (P0.05). PDTC significantly increased GSH (P0.05), decreased MDA and ICAM-1 (P0.05). Conclusion H/R injury damages the function of CAECs mediating by oxidative stress and influences the cellular GSH, MAD and ICAM-1 activities. PDTC effectively protects CAECs from H/R injury may be ascribed to the alleviation of oxidative stress.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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