Title: Study on the optiniization of conditions of soy protein hydrolyzed by papain
Abstract: The effects of temperature,pH,substrate concentration,enzyme concentration on soy protein hydrolyzed by papain were researched with the degree of hydrolysis as indicator through single- factor and orthogonal experiment.The condition was optimized.The result showed that the order of the factors effecting the hydrolyzation was temperature,pH,enzyme concentration and substrate concentration.The optimized parameters were temperature 60℃,pH 7.5,enzyme concentration 3.5%,substrate concentration 5%.Hydrolysis for 40 min under the optimized condition,the degree of hydrolysis of soy protein by papain could reach to 6.58%,the average number of amino acids of soybean peptide was 15,and the average molecular weight was 1875.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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