Title: THE PROTECTIVE EFFECTS OF HIGH PROTEIN DIET ON INTESTINAL BARRIER FUNCTION IN FOOD RESTRICTED RATS
Abstract:Objective: To study the protective role of high protein diet on the intestinal barrier function in food restricted rats. Method: Forty male Wistar rats were randomly divided into five groups: control ...Objective: To study the protective role of high protein diet on the intestinal barrier function in food restricted rats. Method: Forty male Wistar rats were randomly divided into five groups: control group was given 15% casein diet ad-libitum; food restricted group 1 (FR1) and group 2 (FR2) were pair-fed 15% casein diet with 50% and 30% intake of control respecfively; protein supplemented group 1(SP1) and group 2(SP2) were pair-fed 30% casein diet with 50% and 30% intake of control respectively. Each group was fed for 2 w, then blood and intestinal samples were collected to examine the intestinal barrier function. Results: The histomorphology of intestinal mucosa manifested that the damages of intestinal tissue in SP groups were slighter than those in FR groups. The protein and DNA contents in intestinal mucosa of FR groups were lower than those in control group, and tendentiously lower than the corresponding SP groups. The serum diamine oxidase (DAO) activity and D-xylose level in FR group were significantly increased than those in control, but higher than the corresponding SP groups which were higher than control. The DAO activity in intestinal mucosa of SP protein groups was lower than that of control group, but significantly higher than the corresponding FR groups. Conclusion: High protein diet can reduce the damage of intestinal mucosa, and then protect the intestinal barrier function from injury induced by food restriction.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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