Title: Study on the characteristic of polyphenoloxidase and Inhibitor in wax-gourd
Abstract: The activity of wax-gourd polyphenol oxidase(PPO) was spectrophotometer at 420 nm using catechol as substrate.The effects of temperature,pH and inhibitors on PPO were studied and the reaction kinetic equation was established.The results showed that the optimum temperature and pH were 20 ℃ and pH 6.0 respectively.The reaction kinetic equation of enzymatic browning in this experiment built accorded with Michaelis equation modle.The Km,Vmax of kinetic equation were 0.0118 mol/L,0.0586 U/min using catechol as substrate,respectively.The four inhibitors as ascorbic acid,NaHSO3,critic acid and EDTA-2Na had different effects on inhibiting enzymatic browning.These activity of PPO decreased to 25%,32%,62% and 57%.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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