Title: Caloric restriction increases activity of superoxide dismutase in oxidatively damaged SH-SY5Y cells
Abstract: Objective To detect and clarify the superoxide dismutase( SOD) activity in oxidatively damaged human neuroblastoma SH-SY5Y cells cultured under conditions of caloric restriction. Methods SH-SY5Y cells cultured in vitro,were divided into six groups: control group( 3. 0 g / L glucose),H2O2damage( 250 μmol / L) group,low glucose( 2. 0 g/L glucose) group,low glucose + H2O2damage group,resveratrol( 1. 25 μmol / L) group,and resveratrol( 1. 25μmol/L) + H2O2( 250 μmol/L) damage group. The SOD activity of all groups was determined. Results After the pretreatment with low glucose and resveratrol( 1. 25 μmol / L) for 24 hours and treatment with 250 μmol / L H2O2for 1 h,compared with that of the control group,the SOD activity was significantly decreased in the H2O2damage group,significantly increased in the low glucose group,and not significantly changed in the resveratrol( 1. 25 μmol / L) group.Compared with the H2O2damage group,the SOD activity was significantly increased in the low glucose + H2O2group,and slightly increased in the resveratrol + H2O2damage group. After treated with 250 μmol / L H2O2for 7 hours,compared with the control group,the SOD activity was decreased in the H2O2damage group,significantly increased in the low glucose group,and not significantly changed in the resveratrol( 1. 25 μmol / L) group. Compared with the H2O2damage group,the SOD activity was significantly increased in the low glucose + H2O2damage group,and slightly increased in the resveratrol+ H2O2damage group. The SOD activity in the low glucose + H2O2damage group was significantly higher than that in the resveratrol + H2O2damage group. Conclusions Low sugar treatment can improve the SOD activity in cultured cells,suggesting that caloric restriction by increasing the antioxidant enzymes activity in SH-SY5Y cells,reducing oxidation products,decreasing the level of oxidative stress,protects the cells from oxidative damage attacks and slows the occurrence and development of related diseases. Resveratrol does not improve the anti-oxidative damage in the cells through increasing the SOD activity in the culture medium.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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