Title: Determination of Vanillic Acid and Ferulic Acid in Rhizoma Picrorhizae by Capillary Electrophoresis with Electrochemical Detection
Abstract: The determination of vanillic acid and ferulic acid in Rhizoma Picrorhizae by capillary electrophoresis with electrochemical detection(CE-ED)was reported in this paper.The effects of working electrode potential,concentration and pH value of the running buffer,separation voltage and injection time were investigated.Vanillic acid and ferulic acid could be base-line separated in a50mmol/Lboraxbuffer(pH8.4)within8min.A300μm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at potential of0.80V(vs.SCE).There is an excellent linearity between peak current and analytes concentration in the range of5×10 -4 ~1×10 -6 mol/L and1×10 -3 ~1×10 -6 mol/L with the detection limits(S/N=3)of3.0×10 -7 and4.2×10 -7 mol/Lfor vanillic acid and ferulic acid,respectively.The relative standard deviations(RSD)of the peak currents of vanillic acid and ferulic acid were2.2%and2.8%(n=7),respectively.Recoveries of99%for vanillic acid and102.8%for ferulic acid were obtained(n=3).
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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Cited By Count: 2
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