Title: The study of establishing a highly stable and successful diabetic rat model
Abstract: Objective To establish an efficient and stable rat model of hyperglycemia and IR.Methods Wistar rats were fed with high-fat and high-sucrose diet for 4 w followed by 2 times intraperitoneal streptozotocin(STZ) injections.Optimal STZ doses and time intervals were analyzed.Pharmacodynamics and stability test were assessed on the optimized rat model,which were randomly divided into model and metformin treatment groups,and then detected drug effects after 4 w treatment.Rats in model control group(1/2)were continuously fed with normal diet for 8 w,the model's stability was judged.Results Two intraperitoneal STZ injections(25 mg/kg) at the 2-day interval group was demonstrated the highest success rate of model establishment(90%).Two 2-day interval STZ injections(25 mg/kg+m2) showed a 100% success rate of model establishment.According to this modeling method,all rats were met diagnostic criteria for diabetes in terms of fasting and random blood glucose levels and demonstrated increased lipid and glycosylated serum protein levels,decreased insulin levels,as well as abnormal glucose and insulin tolerance.Metformin treatment resulted in effective decrease in blood glucose levels.Rats in the model control group were continuously fed with normal diet for 8 w and their fasting and random blood glucose levels still met diagnostic criteria for diabetes.No mortality was observed.Conclusions Four weeks high-fat high-sucrose diet followed by two 2-day interval STZ injections(25 mg/kg+m2) could be successfully used to establish a rat model of hyperglycemia and IR with 95% efficiency of model establishment and stability.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 1
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