Title: Experiment of insulin treatment on rabbit diabetes mellitus model
Abstract: Objective To investigate the optimal insulin regimens on rabbit diabetes mellitus model according to changes of the islet cell and its apoptosis degree.Methods Rabbit diabetes mellitus models by injection alloxan were divided into 3 groups according to different insulin infusion programs:group A received multiple subcutaneous injection of insulin,group B pre-mixed 50R insulin,group C received pre-mixed 30R insulin.The therapy period was 30 days,the insulin dose and period at the targeted blood glucose level among were observed groups and blood glucose,pathohistology and insulin antibody in un-treatment group and control group were compared.Islets tissue and beta cell apoptosis were marked by hematoxylin-eosin dyeing(HE) and end-labeling(TUNEL).Results Insulin dose of group B[(5.62±0.67)U/kg] is less than those of group A and group C.group C used the largest amount of insulin the targeted blood glucose level.There was significant difference among three groups(P0.01).The period in group A was the shortest[(7.00±1.31)days]and was in group C the longest [(19.63±1.41)days].There was significant difference among three groups(P0.001).Insulin antibody binding rate of group C was significantly higher than those of group B and group C.The damage on beta cells function in group C was the lightest and beta cell apoptosis incidence was the lowest in the same group.The severity degree on islets of langerhans damage and apoptosis in every treated group was ABC.Conclusion Subcutaneous injection of pre-mixed 30R may be the optimal insulin treatment protocols,which supplied more basal insulin and less bolus insulin.Intensive insulin therapy by multiple subcutaneous injection of insulin(triple daily injections short-acting insulin and once daily injection of intermediate-acting insulin or long-acting insulin) was not the recommendation.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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