Title: Cloning and sequence analysis of tobacco antimicrobial peptide genes,novel members of GASA gene superfamily
Abstract: Antimicrobial peptides(AMPs) have been considered to play a key role in plant defense.Three distinct AMP cDNAs(NtSN1a,NtSN1b and NtSN2a) were cloned from tobacco cultivar K326 using in silicon cloning technique,and were analyzed by bioinformatics method.Sequence analysis revealed that both NtSN1a and NtSN1b encoded an open-reading frame of 88 amino acids,and NtSN2a encoded an open-reading frame of 104 amino acids.,all of them shared the conserved domain of the GASA superfamily.Phylogenetic analysis showed that NtSN1a and NtSN1b cluster with potato snaking-1,and NtSN2a clusters with potato snaking-2.These results lay the foundation for clarifying the inhibitory effect of AMPs,discovering their functional differences and understanding the molecular mechanisms underlying tobacco defense responses.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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