Title: Simultaneous determination of the contents of paeonol and paeoniflorin in Danzhi Jiangtang capsule by UPLC
Abstract: Objective: To establish a UPLC method for the simultaneous determination of paeonol and paeoniflorin in Danzhi Jiangtang capsule.Methods: Separation was performed on Waters Acquity UPLC BEH C18(50 mm×2.1 mm,1.7 μm) column by a gradient elution using acetonitrile and 0.1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 0.25 mL·min-1 and a column temperature of 30 ℃.The detection wave length was 230 nm.Results: The linearities of paeonol and paeoniflorin were in the range of 0.653~1.959 μg(r=0.999 7) and 0.108~0.324 μg(r=0.999 9);average recoveries were 99.46%,RSD=0.46%(n=6),99.19%,RSD=0.49%(n=6),respectively.Conclusion: The method is accurate,sensitive,reproducible,and suitable for the quality control of Danzhi Jiangtang capsule.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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