Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Get quick answers to your questions about the article from our AI researcher chatbot

Primary Location

Authors

Topics

Keywords

Related Works

Title: $A study on the regularity of chicken IgY against CHO cell protein and its preparation
Abstract: To explore the regularity of chicken IgY against CHO cell protein and its immunization efforts,and then to prepare chicken IgY of high titer.Leghorns were divided into five groups and were used five different immunization procedures respectively to prepare antibodies.ELISA was applied to detect the regularity of chicken IgY against CHO cell protein.The antibody levels of Leghorns immunized with five different immunization programs are different.Antibody level of quartic low dose intramuscular injection group is significantly lower than that of the quartic high-dose intramuscular injection group(P0.01);Antibody level of triple low-dose intramuscular injection group is significantly lower than that of the triple high-dose intramuscular injection group(P0.01);Antibody level of intramuscular injection group is lower than that of the hypodermic injection group(P0.05).All groups appear antibody peaks at the 20th day after the last immunization.SDS-PAGE analysis showed that the purity of purified IgY is 99% and its ELISA titer is greater than 1∶ 105.From the 28th day after the end immunization,the antibody level of low-dose intramuscular injection group is lower than that of the high-dose intramuscular injection group.And of the same dose,the antibody titer of the intramuscular injection group is lower than that of hypodermic injection group.Chicken IgY of high titer against CHO cell soluble protein has been successfully prepared.