Title: Effects of Fe~(2+) on proliferation and secretion of MMP-2 in umbilical venous smooth muscle cells
Abstract: Objective To investigate the effects of Fe2+on proliferation and secretion of MMP-2 in HUVSMC incubated in high glucose DMEM.Methods Primary cultures of HUVSMCs incubated in the high glucose DMEM(Passage 3~5),were divided into control group and groups in cubated with Fe2+ at five different concentrations(100 mg/L,50 mg/L,25 mg/L,12.5 mg/L,6.25 mg/L).After 24 h of intervention,HUVSMCs proliferation was assessed with MTT method,MMP-2 secretion was measured with ELISA,and the intracellular Ca2+ concentration was assessed by laser confocal microscopy.Results Compared with the control group,HUVSMCs proliferation in 12.5~100 mg/L Fe2+ treatment group was increased(P0.05);the secretion of MMP-2 was promoted in all Fe2+concentration groups(P0.05);and the intracellular Ca2+concentration of HUVSMC was augmented by 100 mg/L Fe2+ treatment(P0.01).Conclusions Fe2+can stimulate the proliferation of VSMC,and increase the intracellular Ca2+ as well as the secretion of MMP-2.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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