Title: Effect of active site of Radix Isatidis on cell apoptosis and expression of IL-10 and TNF-α in RAW264.7 cells
Abstract: Objective To discuss the effect of active sites of Radix Isatidis on apoptosis of Hep-2,Hela,U937,Jukart and on expression of IL-10 and TNF-α in RAW264.7 cells.Methods Hep-2,Hela,U937 and Jukart were pretreated by 0.5 g/L active sites in Radix Isatidis for 24 hours,and the apoptosis were assayed by flow cytometry.U937,infected by HSV-1,was incubated with 0.5 g/L medicine for 24 hours,and the apoptosis were assayed by flow cytometry.The expression of IL-10 and TNF-α in RAW264.7 induced by LPS were detected by ELISA.Results ①The result showed that 0.5 g/L active sites of Radix Isatidis did not affect the apoptosis of Hep-2,Hela,U937 and Jukart,but the apoptosis rate of U937 infected by HSV-1 was reduced from 9.72% to 6.71%,with significant difference between anti-viral group and viral group(P0.01);the G1 phase arrest caused by medicating the Hela cell,and the S phase was shortened,with significant difference between the normal Hela cell and infected Hela cell(P0.05).②In medication group,the apoptosis rates were 19.35%,28.14% and 7.52% after LPS-induced apoptosis in RAW264.7 cells by 1 g/L,0.5 g/L and 0.25 g/L medicine.The apoptosis rates in medication group were lower than that in LPS model group significantly(P0.01).Compared with cell control group,the apoptosis rates in 1 g/L medication group was increased significantly(P0.01),and there was no significant apoptosis promoted effect in 0.5 g/L and 0.25 g/L medication groups.The apoptosis rates in 0.5 g/L and 0.25 g/L medication groups were decreased significantly(P0.01);compared with cell control group,the G1 phase and S phase were shortened,while the G2 phase was extended in 1 g/L medication group,with significant difference between the two groups(P0.01).The G1 phase was extended,but the S phase was shortened in 0.5 g/L medication group significantly(P0.01).③After stimulated RAW264.7 cells by LPS,compared with cell control group,the IL-10 and TNF-α in LPS group were increased significantly(P0.05);the IL-10 in 0.5 g/L and 0.25 g/L medication groups were decreased significantly,and the TNF-α in 1 g/L medication group was increased significantly(P0.05).Compared with LPS model group,the IL-10 in 1 g/L,0.5 g/L and 0.25 g/L medication groups were decreased significantly(P0.05).Conclusion Active sites of Radix Isatidis can inhibited apoptosis in U937 and RAW264.7 cells infected by HSV-1 and LPS.It can decrease the expression of IL-10 in RAW264.7 cells after intervened by LPS for 24 hours.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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