Title: Effects of arsenic on STAT3 m RNA expression in SV-HU-1 cells
Abstract: Objective To explore the effects of arsenite(Na As O2) exposure on the m RNA expression of STAT3 in SV-HU-1cells. Methods Acute and chronic exposure experiments were used in SV-HUC-1 cells in logarithmic growth phase. The cells were treated with Na As O2 at 0,1,2,4,8 and 10 μmol/L respectively for 24 hours in the acute trials, while those which were cultured to the 40 th generation in chronic trials were exposed to 0,0.5 μmol/L sodium arsenite. 0 μmol/L group was regarded as the control group in both experiments. The m RNA expression of STAT3 was measured with RT-PCR. Results Compared with the control group,the expression of STAT3 m RNA in SV-HUC-1 cells increased significantly(P0.05) after exposure to4,8 and 10 μmol/L Na As O2 for 24 hours. And with the increase of sodium arsenite exposure dose, the level of STAT3 m RNA expression in SV-HUC-1 cells was increased at first and then decreased.The significant increasing of STAT3 m RNA expression was also found in SV-HUC-1 cells exposed to 0.5 μmol/L Na As O2 compared with the control group in the chronic trial.Conclusion Arsenic may play an important role in inducing SV-HUC-1 cell malignant transformation through the way of regulating the expression of STAT3.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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