Title: cDNA cloning and protein sequence analysis of chalcone synthase gene in leaves of Ampelopsis grossedentata
Abstract: Objective To clone and analyze the chalcone synthase(CHS) gene sequence from the leaves of Ampelopsis grossedentata.Methods A pair of primers were designed on the basis of the conserved sequences of the cloned plant CHS gene.Using total RNA in the leaves of A.grossedentata as template,the sequence of CHS gene was cloned by reverse transcription polymerase chain reaction(RT-PCR) and then the gene was ligated with pMD18-T Simple vector.The positive clone was sequenced after the identification of clone by PCR.Results A fragment of 1 173 bp was cloned.The sequence analysis indicated that the fragment encoded 390 amino acids and shared sequence homology of more than 67.9% with CHS gene sequences from other higher plants.Conclusion It is the first report that a novel CHS gene is cloned from the leaves of A.grossedentata.This work lays a foundation for the effective application of CHS gene.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 1
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