Title: Effect of glucose on human umbilical vein endothelial cells activity and proliferation
Abstract: Objective To investigate the effect of glucose on human umbilical vein endothelial cells(HUVECs).Methods HUVECs were cultured in vitro with endothelial cell medium.Different dose(10,20,30,40,50 mmol/L) of glucose were added to the HUVECs.Effect of glucose on the HUVECs activity and proliferation were determined by NO content,eNOS activity and cell apoptosis analysis.Results Glucose exhibited dose-dependent ihibitory effect on the HUVECs cell proliferative activity.Glucose could inhibit the effect of eNOs activity and the ability of produce NO on the HUVECs,also exhibited dose-dependence.Conclusions Glucose inhibited the activity and the ability of HUVECs,and the mechanism might be related to up-regulating cell apoptosis and ROS.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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