Title: An experimental study on repair of peripneyal nerve defects with tissue engineering artificial nerve
Abstract: Objective To study the outcome of repair of nerve defects with tissue engineering artificial nerve, which was composed of compound of Schwann cells and biodegradable materials.Methods Extracelluar matrix(ECM) gel (1×10 8/ml ) was fabricated from Schwann cells, which were co cultured with PLA steroframes for 7 days, and implanted into PLA conduits to construct tissue engineering bioartificial nerve . Model of sciatic nerve defects 10mm in length was set up. The animals were divided into 4 groups; artificial nerve group (A),compound of Schawann cells and ECM gel(B), pure ECM gel group(C),autogenous nerve transfer group(D).Evaluation of electrophysiological and histological results was carried out 8 and 12 weeks after operation.Results The regenerating nerve fibers in group A were grown into the distal nerve anastomoses at postoperative 12 weeks. The total myelinated fibers and density of the myelinated fibers in group A were slightly inferior to that of group D .But the regenerated nerve area in group A was larger than that in group D. There was no significant difference in the myelin thickness of sheath and dLAT ?NCV?AMP?AREA between group A and D , but those in group A and D were significantly higher than those in group B and C. Conclusions The tissue engineering bioartificial nerve with compound of Schwann cells,ECM gel,PLA poly hole material and PLA hollow fiber conduit had treatment outcome approximating to that of the nerve autograft for repair of periphral nerve defects.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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