Title: Recombinant Human Papillomavirus Type 6 Virus-like Particles Induced Neutralizing Antibodies
Abstract:Human papillomaviruses(HPVs)are non-enveloped viruses with the capsid composed of major capsid protein L1 and minor capsid protein L2.To investigate the anti-infection effect of immunizing sera raised...Human papillomaviruses(HPVs)are non-enveloped viruses with the capsid composed of major capsid protein L1 and minor capsid protein L2.To investigate the anti-infection effect of immunizing sera raised with recombinant HPV type 6(HPV-6)virus-like particles(VLP),HPV-6 L1 VLP and HPV-6 L1+L2 VLP prepared from h recombinant baculoviruses infected Sf9 cells were used to immunize BALB/c mice.The capability of the raised anti-sera to neutralize virus and inhibit infection was evaluated in cell level and in nude mouse xenograft transplant model after titer determination with ELISA.The titers of serum antibodies in mice vaccinated with both kinds of VLP were above 1∶10,000.The anti-sera specifically inhibited uptake of VLP into human embryonic epithelial cells,and neutralized infection of human foreskins by HPV isolated from genital wart biopsies.In conclusion,the prepared recombinant HPV-6 VLP induced high-titer serum antibodies that could neutralize HPV infection.Recombinant VLP can be used to explore preventive vaccine against genital warts.Read More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot