Title: Correlation between the expression of PTEN and VEGF and angiogenesis in an acute myocardial infarction rabbit model
Abstract: Objective To observe the expressions of phosphatase and tensin homolog deleted from chromosome 10 (PTEN) and vascular endothelial growth factor (VEGF),and explore the correlation between their expressions and angiogenesis in an acute myocardial infarction (AMI) rabbit model. Methods Twenty Japanese rabbits were randomly divided into AMI group (n=15) and sham operation group (SH group,n=5). Samples myocardium were collected from animals of AMI group at 1,2 and 4 weeks post-operation,respectively,from 5 rabbits in each AMI sub-group. The AMI model was reproduced by ligation of left circumflex (LCX) artery,and SH model was reproduced by passing the ligature without actual ligation of the artery. The capillary density (MVD) in the marginal MI area was estimated using Ⅷ factor immunohistochemisty staining. The expressions of VEGF were assayed with immunohistochemisty and QRT-PCR methods,and of PTEN were determined with Western blotting and QRT-PCR methods. Results The expression of VEGF was less,and that of PTEN was higher,in AMI group than in SH group (P0.01). The MVD in the adjacent area of MI was less in AMI 4-week subgroup (45.4±10.0/mm2) than in SH group (103.2±13.3/mm2) (P0.01). The expressions of PTEN protein and PTEN mRNA showed a negative correlation with the expressions of VEGF (r=-0.990,P0.05; r=-0.994,P0.01). Conclusion The increased expression of PTEN in myocardium may inhibit the VEGF expression and accordingly decrease angiogenesis after AMI. Inhibition of PTEN expression may improve the hemoperfusion of the ischemic myocardium.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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