Title: Determination of 3 diaminophenols in hair dyes by ultra performance liquid chromatography tandem mass spectrometry
Abstract: An ultra performance liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometric(UPLC-ESI MS/MS) method has been established for the simultaneous determination of three diaminophenols,including 2,4-diaminophenol dihydrochloride,2,4-diaminoanisole dihydrochloride,and 2,3-Diaminophenol,in hair dyes.The diaminophenols were extracted by water-0.1% formic acid and cleaned up by n-hexane.The separation was carried out by an ACQUITYTM BEH Shield RP18 column using methanol and 5 mmol/L ammonium acetate-0.1% formic acid as the mobile phase.The target compounds were confirmed and quantified by MS-MS under multiple-reaction monitering(MRM) mode with external standard method.Extraction and chromatographic conditions were optimized and the best separation was obtained.Under the optimal conditions,the calibration curves were linear in the range of 10~500 μg/L for the three compounds with correlation coefficients more than 0.998.The limits of detection(LOD) were 3 μg/kg,and the limits of quantitation(LOQ) were 10 μg/kg.The average recoveries of 3 diaminophenols were between 80.3% and 113.5% with the relative standard deviations(RSDs) no more than 8.6%.The proposed method was effective,simple and sensitive,and was suitable for the determination and confirmation of diaminophenols in hair dyes.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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