Title: The effects of protein kinase C on the uptake and distribution of adriamycin in the multidrug resistant LoVo/Adr cells of colorectal carcinoma
Abstract: Objective To observe the effects of protein kinase C on the uptake and distribution of ADR in the multidrug resistant LoVo/Adr cells of colo rectal carcinoma. Methods The uptake of ADR in cells was studied by flow cytometry and the distribution of ADR in cells was examined by fluorescent microscope. The expression of P gp was detected by immunohistochenical method. Results P gp stain was negative in LoVo cells, but intensively positive in LoVo/ADR cells. The concentration of ADR was in LoVo/Adr cells was significantly lower than that in LoVo cells. The uptake of ADR was reduced after treating with PMA in LoVo/Adr cells, but was significantly increased after treating with SP. The fluorescence of ADR in the drug sensitive LoVo cells was mainly distributed in the nucleus, but only a little in the cytoplasm. While the distribution of ADR in LoVo/Adr cells markedly decreased in the nucleus and relatively increased in the cytoplasm. Neither PMA nor SP could change the distribution of ADR in LoVo/Adr cells. Conclusion Not only the uptake of ADR decreased but also the distribution of ADR was abnormal in resistant cells. PKC might modify multidrug drug resistance by effecting on the uptake but not on the distribution of ADR in resistance cells.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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