Title: Study on Technology of Tissue Culture and Rapid Propagation of Acacia cincinnata
Abstract: The tissue culture and rapid propagation technology researches of generation-beginning culture,the generation-continuing seedling multiplication culture,rooting culture and culture seedling transplanting were conducted with Acacia cincinnata plus tree stem with buds as explant.The results revealed: the suitable flow of the explant generation-beginning culture was following: the spikes on the mid and upper locations of crown were treated by 70% of alcohol for 40s,then were sterilized with 0.1% of HgCl2 for 8 min.The germination rate reached 35.7%;generation-continuing culture optimal culture medium was: the improved MS+6-BA 0.8 mg·L-1+IAA0.5 mg·L-1+sugar 30 g·L-1,the multiplication multiple reached 5.2 times.The rooting culture was conducted with generation-continuing bud seedling height of 1.1 to 1.4 cm and the best medium was 1/2MS +IBA1.5 mg·L-1+ABT1# 0.2 mg·L-1+NAA 0.2 mg·L-1,the highest rooting rate reached 97.8%;the culture plant was transplanted with the best medium of deep red soil+peaty soil(3∶1),the survival rate reached 90%.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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