Title: Cloning and Sequence Analysis of IFN-γ Gene Gene in Guangxi Water Buffalo
Abstract: Total RNA was isolated from cultured water buffalo peripheral blood lymphocytes stimulated with ConA.The cDNA of water buffalo interferon-γ(IFN-γ) cDNA was amplified by RT-PCR,and then cloned into pMD18-T and sequenced.The ORF of the cloned water buffalo IFN-γ gene was 501bp in length,encoding a mature polypeptide with 166 amino acids.Compared with other IFN-γ,the nucleotide homologies between the water buffalo IFN-γ gene and the cattle IFN-γ genes were higher than 97%,the amino acid homologies were higher than 96%.The homologies with other animals were step down in accordance with its genetic relationship.The results showed that the cloning plasmid was successfully constructed,and it is the basis for further study and application of Guangxi water buffalo IFN-γ.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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