Title: Regulatory role of MAPK signaling pathway in the change of cytokines of Jurkat T cells induced by PM2.5
Abstract: Objective To explore the regulatory role of MAPK signaling pathway in the change of cytokines of Jurkat T cells induced by traffic-related PM2.5. Methods Expressions of the phosphorylation of JNK, P38MAPK and AP-1 protein in Jurkat T cells stimulated by traffic related PM2.5 for different time were determined by Western-blot method, and the levels of TNF- α and IL-1β in cells supernatant were detected by ELISA method. Results After 320 μg/ml of PM2.5 stimulated Jurkat T cells for 24h,p-JNK/β-actin gray level ratio was significantly higher than that in the normal saline group, and the difference was statistically significant. P-p38MAPK/β-actin intensity ratios in 80μg/ml and 320 μg/ml of PM2.5 groups were higher than those in the saline group, the difference was statistically significant(P0.05). All exposed groups respectively with the p-JNK antagonist SP600125 or p-p38MAPK antagonist SB2035 80, p-JNK/β-actin and p-p38MAPK/β-actin gray ratio reduced than normal exposure groups,especially at 320 μg/ml of PM2.5 group. After 80 μg/ml of PM2.5 stimulated cells for 6h and 24h, AP-1/β-actin protein gray ratio,IL-1βlevels of cell supernatant were higher than normal saline group, the difference was significant(P0.05). After 80 μg/ml of PM2.5 stimulated cell for 3h, 6h and 24h, TNF- α levels of cell supernatant increased gradually, had obvious time effect trend, and there was a significant difference in the normal saline group(P0.05). Conclusion Traffic-related PM2.5 can cause Jurkat T cells to release more inflammation cytokines TNF-α and IL-1β, p38MAPK and JNK regulate the release of TNF- α and IL-1βthrough AP-1.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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