Title: Tissue culture and plant regeneration in Begonia fimbristipula
Abstract: An efficient and rapid regeneration protocol was developed using leaves from geminating seedlings of Begonia fimbristipula.MS medium containing 1.00 mg/L 2,4-D,1.0 mg/L 6-BA,200 mg/L LH,200 mg/L CH and YE 200 mg/L was most effective in inducing calli.The frequency of callus induction was above 96.88%.Optimal medium for inducing adventitious buds is MS+1.0 mg/L 6-BA+200 mg/L LH+200 mg/L CH+YE 200 mg/L, and the highest frequency reached 77.3%.The proper culture medium of rooting was 1/2MS and the rooting rate was 100%.The survival rate of the plantlet was up to 90%after being transplanted into soil.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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