Title: Construction of eukaryotic expression vector for the long type of human LRP16 gene and its expression in HL60 leukemia cell line
Abstract: Objective:To construct an eukaryotic expression vector containing the open reading frame (ORF) of long type of LRP16 gene and to detect its expression in HL60 cells.Methods:The long type of LRP16 gene was ligated with the cloning vector pGEM-T, and then the identified fragment was recombined with the eukaryotic expression vector pcDNA3.1 (+). HL60 cells were transfected with the recombinant pcDNA3.1 (+)-long type LRP16 by Superfect. The expression of long type LRP16 in the transfected HL60 cells was assayed by RT-PCR.Results and Conclusion:Restriction analysis and DNA sequencing showed that the recombinant plasmid contained the ORF of long type LRP16 gene. Transfection experiment verified that LRP16 gene could be expressed in HL60 cells, which is the basis for the further study of LRP16.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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