Title: OPTIMIZATION OF XYLANASE PRODUCTION FROM ASPERGILLUS ORYZAE BY USING AGRICULTURAL WASTES IN SUBMERGED FERMENTATION
Abstract: Cultural conditions for xylanase production by the strain Aspergillus oryzae M-9 were optimized under submerged fermentation.The strain secretes high levels of xylanase on the agricultural residues of Corn cobs as well as on xylans.The results of single-factor experiment showed that 0.08mm Corn cobs(4.0%) was the best carbon source and inducer.A combination of yeast extract(1.2%) and soybean peptone(0.8%) was the best nitrogen source.The optimal initial pH value,cultivation temperature and the rotation rate of the cultures were pH 7.5,30℃ and 200r/min,respectively.The maximum increase in xylanase production was observed in the surfactants of Tween 60.Under the optimized conditions,the highest xylanase activity of 795.93U/mL was obtained in the 5th day.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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