Title: Gliquidone-stimulated insulin secretion features are different from glibenclamide in HIT-T15 cells
Abstract: Objective To determine whether gliquidone has any different effect on the insulinsecretory profiles of HIT-T15 cells from gliclazide and glibenclamide in response to glucose stimuli. Methods HIT-T15 cells (β-cells line) were cultured for 8h in RPMI-1640 with three different glucose concentrations (5,10,15mmol/1),the cells were re-cultured with gliquidone,gliclazide and glibenclamide in physiological concentrations,and then,insulin levels in the medium at 0,10,20,30,45,60,120 and 180 minutes were measured by radiommunoassay (RIA).Results In this study,all three sulfonylureas strongly stimulated the insulin release in various concentrations in the present of glucose.Gliquidone and gliclazide induced a similarly rapid biphasic insulin secretion,reaching the first peak at 10 minutes and the second lower peak at 30 minutes,followed by gradual decline to near basal values at the 180 minutes.In contrast,glibenclamide induced a strong but monophasic insulin release,reaching its peak at 45 minutes followed by somewhat decline of the hormone release but did not return to basal values at 180 minutes. The undercurve area of insulin secretion was significantly smaller in gliquidone than in glibenclamide. Conclusions Gliquidone stimulates insulin release of HIT-T15 cells in biphasic manner which is similar to glielazide,but different from glibenclamide displaying monophasic pattern.This unique feature in insulin secretion of gliquidone may have important implications in its clinical use.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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