Title: A Preliminary Study on Inhibitory Effect of IL-24 in Nude Mice Model of Cervical cancer
Abstract: Objective To study he inhibition function of gene recombinant plasmid pDC316-hIL-24 on human cervical cancer nude mice model tumor growth and invasion.Method Nude mice xenograft models were established with human cervical cancer Hela cells,and then animal models were randomly divided into three groups,each group had 6 mice: PBS(groupⅠ),empty plasmids(group Ⅱ),recombinant plasmids pDC316-hIL-24(group Ⅲ).All the nude mice were injected into the tumor-bearing mice with Lipofectamine 2000 to interfere with the growth of transplanted tumor.Their effects on the volume of the tumor and inhibitory rate of the mice were observed.hIL-24 expression was determined in transplanted tumor by PCR.Lymph node metastasis was observed,and as well as immunohistochemistry was employed for investigating nm23H1 albumen in each group.Results ①After transfection IL-24 gene was successfully transfected into and expressed in tumor cells.Its function of inhibiting tumor growth was 42.9%,significantly higher than PBS,empty plasmids(P0.05).②Lymph node metastasis was decreased in group Ⅲ(P0.05).Immunohistochemistry showed that,the expression of nm23H1 albumen in IL-24 group was significantly higher than other two groups(P0.05).Conclusion Gene recombinant plasmid pDC316-hIL-24 can significantly inhibit the growth of transplanted human cervical cancer in nude mice.IL-24 may activate expression of nm23H1 albumen to inhibit human cervical cancer nude mice model tumor growth and invasion.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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