Title: Fabrication of Diagnostic microarray for Subtyping Avian Influenza Virus
Abstract: To develop the diagnostic microarray for detection of the AIV haemagglutinin subtypes H5,H7 and H9 and neuraminidase subtypes N1 and N2 the probe genes of H5,H7,H9,N1,N2 and control gene of GAPDH were amplified by PCR with recombinant plasmids templates respectively and precipitated by isopropanol.They were diluted to 0.3μg/μl and spotted onto aldehyde glass slide with microarray printing system.The spot space was 450μm .The diameter of spot was 220μm .The spotted slide was dried at room temperature for at least 24h,hydrated for 10s at 65℃.UV cross-linking was carried out by placing the slides, in a crosslinker, exposing the slides to 65mJ of energy.After blocking and denaturation,the diagnostic gene chip for detecting H5,H7,H9,N1,N2 were produced successfully.Prepared samples were labelled by PCR with fluorescence CY3 for testing its qualification.The results of qualification test showed that the diagnostics are qulificated and can be used to detect some subtypes of avian influenza virus at the same time.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot