Title: Establishment of Agrobacterium-mediated transformation system of strawberry with chitinase gene
Abstract:With the transformation strawberry leaves,transgenic strawberry was obtained through the chit42 gene transformation by Agrobacterium tumefactions,so that disease-resistance transgenic plants were requ...With the transformation strawberry leaves,transgenic strawberry was obtained through the chit42 gene transformation by Agrobacterium tumefactions,so that disease-resistance transgenic plants were required.Factors on Agrobacterium tumifaciens-mediated gene transfer was discussed in details.We preliminarily determined an effective system for genetic transformation.The protocol was follows: AS 80~120 μmol/L as co-culture medium,10~20 minutes for infection,3 days for co-culture.The regenerated kanamycin resistant plants were analyzed by PCR.The result indicated that the chit42 gene has been integrated into the strawberry.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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