Title: Influence of Buguzhi Compound on Biological Behavior of Stomach Cancer Cell SGC7901
Abstract:Objective:To investigate the effects of Buguzhi compound on proliferation、adhesion and invasion of gastric carcinoma cell line SGC7901 in vitro.To investigate the effects of Buguzhi compound on apopto...Objective:To investigate the effects of Buguzhi compound on proliferation、adhesion and invasion of gastric carcinoma cell line SGC7901 in vitro.To investigate the effects of Buguzhi compound on apoptosis of gastric carcinoma cell line SGC7901.Methods:After gastric carcinoma cell line SGC7901 was treated with Buguzhi compound in different concentrations,cell proliferation and adhesion capabilities were assessed using MTT assay.The invasion capabilitie was assessed using Transwell method.The apoptosis rate was examined by TUNEL method.Results:(1)Buguzhi compound significantly inhibited the proliferation of SGC7901 cells in a concentration-dependent manner(P0.05).As compared with the control group,the adhesion capabilities were significantly inhibited by high-concentration group(P0.05);inhibited by moderate-concentration group after 90min(P0.05);inhibited by low-concentration group after 120min(P0.05).The cell invasion inhibition rate of high-concentration group、moderate-concentration group and low-concentration group were 60.79%(P0.01),38.81%(P0.01)and 8.7% respectively.(3)The apoptosis rate of high-concentration grou、moderate-concentration group and low-concentration group were 59.08%(P0.01)、31.45%(P0.01)and 16.6%,respectively.Conclusion:(1)Buguzhi compound can inhibite the proliferation of SGC7901.(2)Buguzhi compound can significantly inhibit adhesion and invasion capabilities of SGC7901.(3)Buguzhi compound can significantly induce apoptosis.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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