Title: Effects of IFN-γ on ATP Binding Cassette Transporter A1 Expression and Cholesterol Efflux in THP-1 Macrophage-derived Foam Cells
Abstract: In order to study the effect of IFN γ on ATP binding cassette transporter A1(ABCA1) expression and cholesterol efflux in THP 1 macrophage derived foam cells, after exposure of the cultured THP 1 macrophage derived foam cells to IFN γ for different time, cholesterol efflux and ABCA1 mRNA and protein level were determined by FJ 2107P type liquid scintillator and reverse trancriptase polymerase chain reaction(RT PCR) and Western blot, respectively and the mean ABCA1 fluorescence intensity on THP 1 macrophage derived foam cells was detected by flow cytometry. It was demonstrated that exposure of the cultured THP 1 macrophage derived foam cells to IFN γ for different time results in decreasing cholesterol efflux, the expression of ABCA1 mRNA and protein and the mean ABCA1 fluorescence intensity in THP 1 macrophage derived foam cells in a time dependent manner. Exposure of the cultured THP 1 macrophage derived foam cells to IFN γ for different time results in increasing total cholesterol, free cholesterol and cholesterol ester in THP 1 macrophage derived foam cells in a time dependent manner. It can be concluded that IFN γ decreases cholesterol efflux and the expression of ABCA1 in THP 1 macrophage derived foam cells.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot