Title: Detection of spermatogenic cells in semen and analysis on Y chromosome microdeletion in patients with non-gomphosis Klinefelter syndrome
Abstract:Objective:To analyze the detection results of spermatogenic cells in semen and the microdeletion condition of azoospermia factor of Y chromosome in patients with Klinefelter syndrome. Methods:27 cases...Objective:To analyze the detection results of spermatogenic cells in semen and the microdeletion condition of azoospermia factor of Y chromosome in patients with Klinefelter syndrome. Methods:27 cases with non-gomphosis Klinefelter syndrome and 27 normal men were selected,the levels of serum follicle stimulating hormone(FSH),luteinizing hormone(LH) and testosterone were detected,chromosome karyotyping was performed;and their semen samples received detection of spermatogenic cells;polymerase chain reaction(PCR) was performed to screen Y chromosome microdeletion. Results:The serum levels of FSH and LH in non-gomphosis Klinefelter syndrome group were significantly higher than those in control group,but the serum level of testosterone in non-gomphosis Klinefelter syndrome group was significantly lower than that in control group;the results of spermatogenic cells detection showed that one case was at sperm level,one case was at primary spermatocyte level,8 cases were at rare sperm level;the other 17 cases had no sperm and spermatogenic cell at all levels in their semen samples;sperms and spermatogenic cells at all levels could be found in semen samples of control group;no azoospermia factor microdeletion of Y chromosome was found in the two groups. Conclusion:Seminal spermatogenic cells examination can reflect the spermatogenesis in testis of patients with non-gomphosis Klinefelter syndrome,which can provide reference for clinical diagnosis before assisted reproduction;azoospermia factor microdeletion of Y chromosome may be not one of the genetic factors inducing dyszoospermia of patients with non-gomphosis Klinefelter syndrome,the relationship between azoospermia factor microdeletion and Klinefelter syndrome is uncertain.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot