Title: Effects of acute denervation on ratio change of nicotinic acetylcholine receptor subtypes in the rat gastrocnemius muscle
Abstract: Objective To identify the effects of acute denervation on time-dependent change in mRNA and protein expression of adult-type(e-AChR) and embryonic-type(γ-AChR) nicotinic acetylcholine receptor in rat gastrocnemius muscle.Methods Sprague-Dawley rats were divided into the control group(Group C) and the denervated group(Group D).Rats in group D were sacrificed randomly on the 7th(Group D7),14th(Group D14),21st(Group D21),28th(Group D28) and 35th day(Group D35) after severing the left sciatic nerve.Messenger RNA(mRNA) and protein expression of e-AChR and γ-AChR in gastrocnemius muscle from both hinder limbs was measured by RT-PCR and Western blotting respectively to detect the ratio change in expression of e-AChR and γ-AChR.Results The total AChR expression increased significantly from the 14th day and maintained at a high level.e-AChR expression first decreased within 7 days after denervation and then increased significantly from the 14th day after denervation while γ-AChR increased constantly,and reached a peak percentage on the 7th day.Conclusion Time-dependent expression changes in e-AChR and γ-AChR exists obviously after denervation,which may affect the denervated muscle on its sensitivity to muscle relaxants.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 1
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