Title: RAPD analysis on inter-species relationships in Poa
Abstract: Ten species of Poa were analyzed using random amplified polymorphic DNA(RAPD) markers. 30 decamer oligonucleotide random primers were used for polymorphic section, among which, 28 primers produced polymorphic RAPD products. A total of 250 bands amplified from 18 primers were selected for RAPD analysis. Jaccard's genetic similarity coefficients were generated and dendrogram was constructed using UPGMA. The result showed that genetic distance between P. botryoides and P. psilolepis was the least, with coefficient 6.7; then the one between P. sinattenuata and P. sphondylodes, with coefficient 6.8; while the genetic distance between P. sikkimensis and P. pratensis, P. sikkimensis and P. psilolepis showed much farther, with coefficient 10.5. This is similar to morphological taxonomy result, so RAPD is a useful supplementary method in assessing genetic relationship among Poa species.
Publication Year: 2002
Publication Date: 2002-01-01
Language: en
Type: article
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Cited By Count: 2
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