Title: Prokaryotic Expression and Purification of Mouse Recombinant Dendritic Cell-derived Factor 1-DCF1
Abstract: Objective:To clone,express and purify mouse recombinant dendritic cell-derived factor 1-DCF1.Method:dcf1 gene was amplified from mouse brain cDNA by PCR,and subcloned into prokaryotic expression vector pET30a.This recombinant plasmid was transformed into E.coli BL21(DE3).Recombinant DCF1 expression was induced by IPTG.Resulted protein was purified by Ni sepharose ff6 resin under denature condition.SDS-PAGE and Western blot method were used to identify the expression and purification of recombinant DCF1.Result:Mouse dcf1 cDNA fragment of 972bp was obtained,and was subcloned into prokaryotic expression vector pET30a.A 42kDa recombinant DCF1 was successfully induced to express in E.coli and further purified by Ni sepharose FF6 affinity chromatograph.Conclusion:A method for prokaryotic expression and purification of mouse recombinant DCF1 was successfully established.This method laid a foundation for the subsequent DCF1 research.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot