Title: Analysis of bacterial diversity in deep-sea sediments from Pacific polymetallic nodule province
Abstract: The environmental total DNAs were directly extracted from two sediment samples from Pacific polymetallic nodule province(112.950 3°W,11.884 9°N,water depth of 4 160 m;145.351 4°W,8.374 0°N,water depth of 5 257 m).Two bacterial 16S rRNA gene libraries were generated by using PCR and TA cloning.Phylogenetic results indicated that 93 clones could be divided into 13 phylotypes,including Alphaproteobacteria,Gammaproteobacteria,Deltaproteobacteria,Betaproteobacteria,Planctomycetes,Acidobacteria,Cytophaga-Flavobacterium-Bacteroidetes(CFB),Nitrospira,Actinobacteria,Chloroflex,Firmicutes,Deinococcus-Thermus,and candidate division OP11.16S rDNA sequences from members of Gammaproteobacteria dominated two sediments samples.Proteobacteria(Alpha-,Gamma-,Beta-,Delta-),Planctomycetes,Acidobacteria,CFB,Nitrospira appeared in two sediments samples.DOTUR and LIBSHUFF statistics of 16S rDNA gene sequences from the two libraries revealed major differences,indicating a high richness in the sediment bacterial communities and considerable variability in bacterial community composition among regions.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot