Title: Inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro
Abstract:Objective To investigate the inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro. Methods Rat glioma C6 cells were cultured with different ...Objective To investigate the inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro. Methods Rat glioma C6 cells were cultured with different concentrations of proteasome inhibitor MG-132 (10,20 and 50 μmol·L-1).Cell viability was determined by MTT assay at different cultured periods.Flow cytometry was used to detect apoptosis change and HE and AO/EB staining and electronic microscope were used to detect the morphological changes of apoptotic cells.Results Compared with normal control,MG-132 significantly reduced the viability of C6 cells in 10,20 and 50 μmol·L-1MG-132 groups (P0.01).Moreover,the inhibitory effect was higher on high concentration MG-132 compared with low dose MG-132. After treatment with 10 μmol·L-1MG-132 for 24 h,the apoptosis characteristic C6 cells were detected by AO/EB and HE staining.Apoptotic sub-G was detected by flow cytometry in C6 cells incubated with 10 μmol·L-1 MG-132 for 12 h and displayed a time-dependent manner,the apoptotic percentages in 10,20 and 50 μmol·L-1 MG-132 groups were significantly higher than that in control group (P0.01).Conclusion Proteasome inhibitor MG-132 can inhibit C6 cell proliferation and induce C6 cell apoptosis.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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