Title: ESTABLISHMENT OF HBV AND HCV GENOTYPING AND CO-DIAGNOSIS METHOD WITH GENE CHIP TECHNIQUE
Abstract: [Objective]To establish genotyping and diagnosis methods for hepatitis B virus(HBV) and hepatitis C virus(HCV)using gene chip technique.[Methods]Primers and genotyping probes were designed in region X and region pro-C of HBV and 5'-untranslated region of HCV.Primers were biotin-labeled to amplify HBV DNA and HCV cDNA.Genotyping probes were immobilized on nylon membrane to make genotyping gene chip.The PCR products were hybridized with the genotyping gene chip.The color was developed adding POD and TMB.A judgment could be done certainly according to position of color reaction.The reliability of this new method was verified by FQ-PCR and sequencing.HBV and HCV infection could be identified and genotyped with the method we established simutaneously.30 HBV DNA-positive,30 HCV RNA-positive sera and 5 HBV/HCV coinfection sera were genotyped.[Results]The results of genotyping gene chip were coincided with FQ-PCR and sequence analysis.[Conclusion]HBV and HCV co-diagnosis and genotyping can be realized by gene chip technique which has proved to be sensitive,specific,precise and economic,and it is suitable in clinical practice and epidemic study.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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