Title: Determination of paeoniflorin concentration in mice plasma after oral administration of Chishao Wuzhuyu fufang by RP-HPLC
Abstract: OBJECTIVE To determine paeoniflorin in mice plasma. METHODS An HPLC method was adopted.The conditions of chromatography:A C 18 radial compression column (250 mm×4.6 mm,7 μm) was used with a mobile phase of CH 3OH-H 2O (38∶62). The detectve wavelength was 230 nm and flow rate was 0.5 ml·min -1. External standard method was used for quantitative analysis.RESULTS The linear range was 5.0-250.0 ng·μl -1(r=0.9999). The minimum detection limit was 1.49 ng·μl -1,the average recovery was 95.52%. CONCLUSION This method provides an accurate and sensitive way in detecting plasma concentration of paeoniflorin.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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