Publication Information

{'id': 'https://openalex.org/W2353427752', 'doi': None, 'title': 'Preparation and Purification of Anti-human DR5 Polyclonal Antibody', 'display_name': 'Preparation and Purification of Anti-human DR5 Polyclonal Antibody', 'publication_year': 2008, 'publication_date': '2008-01-01', 'ids': {'openalex': 'https://openalex.org/W2353427752', 'mag': '2353427752'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTotal-SWJS200806011.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S192932257', 'display_name': 'Biotechnology(Faisalabad)', 'issn_l': '1682-296X', 'issn': ['1682-296X', '1682-2978'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4322697005', 'host_organization_name': 'Asian Network for Scientific Information', 'host_organization_lineage': ['https://openalex.org/P4322697005'], 'host_organization_lineage_names': ['Asian Network for Scientific Information'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5057555537', 'display_name': 'Fei Chang', 'orcid': 'https://orcid.org/0000-0002-3724-2455'}, 'institutions': [{'id': 'https://openalex.org/I2722730', 'display_name': 'Inner Mongolia University', 'ror': 'https://ror.org/0106qb496', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I2722730']}], 'countries': ['CN'], 'is_corresponding': True, 'raw_author_name': 'Chang Fei', 'raw_affiliation_strings': ['Department of Biology,College of Life Science,Inner Mongolia University,Hohhot 010021,China)'], 'affiliations': [{'raw_affiliation_string': 'Department of Biology,College of Life Science,Inner Mongolia University,Hohhot 010021,China)', 'institution_ids': ['https://openalex.org/I2722730']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': ['https://openalex.org/A5057555537'], 'corresponding_institution_ids': ['https://openalex.org/I2722730'], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 63}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12996', 'display_name': 'Cancer Research and Treatments', 'score': 0.9336, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12996', 'display_name': 'Cancer Research and Treatments', 'score': 0.9336, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11041', 'display_name': 'Ubiquitin and proteasome pathways', 'score': 0.9064, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/polyclonal-antibodies', 'display_name': 'Polyclonal antibodies', 'score': 0.97179496}], 'concepts': [{'id': 'https://openalex.org/C956191', 'wikidata': 'https://www.wikidata.org/wiki/Q930144', 'display_name': 'Polyclonal antibodies', 'level': 3, 'score': 0.97179496}, {'id': 'https://openalex.org/C91523082', 'wikidata': 'https://www.wikidata.org/wiki/Q420496', 'display_name': 'Antiserum', 'level': 3, 'score': 0.93149734}, {'id': 'https://openalex.org/C123894998', 'wikidata': 'https://www.wikidata.org/wiki/Q425158', 'display_name': 'Fusion protein', 'level': 4, 'score': 0.8617376}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.73216975}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.6885347}, {'id': 'https://openalex.org/C32611913', 'wikidata': 'https://www.wikidata.org/wiki/Q2166189', 'display_name': 'Titer', 'level': 3, 'score': 0.6795858}, {'id': 'https://openalex.org/C85265743', 'wikidata': 'https://www.wikidata.org/wiki/Q885458', 'display_name': 'Blot', 'level': 3, 'score': 0.65690786}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.6191475}, {'id': 'https://openalex.org/C179247698', 'wikidata': 'https://www.wikidata.org/wiki/Q382799', 'display_name': 'Affinity chromatography', 'level': 3, 'score': 0.43032455}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.42894506}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.39058888}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.1955525}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.07438651}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.069389194}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.060877502}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'http://en.cnki.com.cn/Article_en/CJFDTotal-SWJS200806011.htm', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S192932257', 'display_name': 'Biotechnology(Faisalabad)', 'issn_l': '1682-296X', 'issn': ['1682-296X', '1682-2978'], 'is_oa': True, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4322697005', 'host_organization_name': 'Asian Network for Scientific Information', 'host_organization_lineage': ['https://openalex.org/P4322697005'], 'host_organization_lineage_names': ['Asian Network for Scientific Information'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 0, 'referenced_works': [], 'related_works': ['https://openalex.org/W2387788983', 'https://openalex.org/W2386955804', 'https://openalex.org/W2381341121', 'https://openalex.org/W2376497954', 'https://openalex.org/W2375047767', 'https://openalex.org/W2373789428', 'https://openalex.org/W2372224849', 'https://openalex.org/W2372135463', 'https://openalex.org/W2369321476', 'https://openalex.org/W2364487344', 'https://openalex.org/W2362491927', 'https://openalex.org/W2361558405', 'https://openalex.org/W2360587303', 'https://openalex.org/W2357207051', 'https://openalex.org/W2354244512', 'https://openalex.org/W2351434051', 'https://openalex.org/W2351052967', 'https://openalex.org/W2350254841', 'https://openalex.org/W2102372801', 'https://openalex.org/W2011309130'], 'abstract_inverted_index': {'Objective:': [0], 'To': [1], 'purify': [2], 'GST-eDR5': [3, 16, 28, 39, 67, 113], 'fusion': [4, 29, 40, 65, 114], 'protein': [5, 15, 30, 66], 'and': [6, 23, 50, 60, 70, 84, 103, 110, 123, 138], 'prepare': [7], 'the': [8, 37, 111], 'mouse': [9, 35, 78], 'anti-human': [10, 79], 'DR5': [11, 80, 107], 'antisera.Method:': [12], 'The': [13, 116], 'recombinant': [14, 112], 'was': [17, 31, 43, 68, 82, 90], 'purified': [18, 38, 44, 64, 96], 'by': [19, 33, 45, 57, 86, 97], 'GST': [20, 98, 102], 'purification': [21], 'kit': [22], 'PAGE.The': [24], 'polyclonal': [25, 53, 117], 'antibody': [26, 54, 119], 'against': [27], 'prepared': [32], 'immunizing': [34], 'with': [36, 47, 120], 'protein,and': [41], 'then': [42], 'reaction': [46], 'GST.The': [48], 'specificity': [49, 76, 122], 'titer': [51, 89, 125], 'of': [52, 77], 'were': [55], 'detected': [56], 'western': [58, 87], 'blotting': [59], 'indirect': [61], 'ELISA.Result:': [62], 'High': [63], 'obtained': [69], 'its': [71, 136], 'concentration': [72], 'is': [73], '0.65μg/μl.Very': [74], 'high': [75, 92, 121, 124], 'antiserum': [81], 'generated': [83, 128], 'analyzed': [85], 'blotting.The': [88], 'as': [91, 93], '1∶25600.The': [94], 'antisera': [95], 'fails': [99], 'to': [100], 'recognize': [101, 105], 'only': [104], 'native': [106], 'from': [108], 'cells': [109], 'protein.Conclusion:': [115], 'anti-DR5': [118], 'has': [126], 'been': [127], 'successfully.This': [129], 'study': [130], 'settles': [131], 'a': [132], 'foundation': [133], 'for': [134], 'investigating': [135], 'inhibition': [137], 'apoptosis': [139], 'effect': [140], 'on': [141], 'tumor': [142], 'cells.': [143]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2353427752', 'counts_by_year': [], 'updated_date': '2024-12-11T15:08:18.088487', 'created_date': '2016-06-24'}