Title: Experimental Study of Berbamine Inducing Apoptosis in Human Leukemia Jurkat Cells
Abstract: [Purpose] To explore the mechanism of berbamine inducing apoptosis in human leukemia Jurkat cells. [Methods] IC50 value and cytotoxity of human Jurkat cells were detected by MTT method; apoptotic body, by fluorescent staining with Hoechst33258; cell apoptotic rate, by FCM with Annexin-Ⅴ FITC-PI staining method; apoptotic peak, cell cycle and protein expression of caspase-3, by FCM. [Results] Berbamine could significantly inhibit the proliferation of Jurkat cells in a dose-dependent manner and the IC50 value was 6.6±0.5μg/ml at 48 hours. Berbamine also selectively induced apoptosis of Jurkat cells which were arrested in the S phase. In addition, expression of caspase-3 protein of Jurkat cells increased after exposure to berbamine. [Conclusions] Berbamine can selectively induce apoptosis of human leukemia Jurkat cells in the manner of dose and time dependence through up-regulation expression level of caspase-3 protein.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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