Title: The regulatory effects of dexamethasone on the expression of bcl 2 mRNA in neonatal rats following cerebral hypoxia ischemia
Abstract: Objective To investigate the possible mechanism of the
neuroprotective effect of dexamethasone (DEX) on neonatal rats following cerebral hypoxia
ischemia. Methods Rapid competitive reverse transcriptase PCR was used to analyse the
expression of bcl 2 mRNA at ipsilateral cerebral hemisphere semi quantitatively in neonatal
rat models of hypoxia ischemia, pre treatment with DEX prior to hypoxia ischemia; treatment
with DEX immediately following hypoxia ischemia; pre treatment with DEX prior to sham and
normal controls, respectively. Results The expression of bcl 2 mRNA in the ipsilateral
hemisphere following cerebral hypoxia ischemia, began at 6h after recovery, peaked at 12h,
decreased to lower level at 24h, and returned towards baseline at 72h (n=3/time point). The
expression of bcl 2 mRNA in the ipsilateral hemisphere induced by hypoxia ischemia could be
inhibited by pretreatment with DEX prior to hypoxia ischemia at the early stage of recovery, but
the induction of bcl 2 mRNA began at 24h, and lasted at least 7 days after recovery. DEX
administered to the rats immediately following hypoxia ischemia had no effect on the
expression of bcl 2 mRNA. Conclusion Pretreatment with DEX exerts neuroprotective effect on
neonatal rats following hypoxia ischemia, partially through positive induction of the expression
of bcl 2 mRNA.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot