Title: Relationship between the cell cycle regulation factors and clinicopathologic features and prognosis of colorectal carcinoma
Abstract: Objective To detect the correlation of cell cycle regulation factors Cyclin D1, CDK4, P16 protein and p16 gene expression with clinicopathologic features and prognosis in colorectal carcinoma. Methods The expression of Cyclin D1, CDK4 and P16 protein were examined with SP immunohistochemical technique in 89 cases of colorectal carcinoma and 12 cases of normal colorectal tissues, p16 gene was detected with SSCP-PCR technique in 40 cases of colorectal carcinoma. The relationship was analyzed among the Cyclin D1, CDK4, P16 protein and p16 gene expression with clinical stage, pathological grade, lymph node metastasis and survival rate of patients with colorectal carcinoma. Results The positive staining was located at nucleus for Cyclin D1, cytoplasm and nucleus for P16 and CDK4 in colorectal carcinoma. The positive staining was only located within cytoplasm for P16 and CDK4, and no positive staining for Cyclin D1 in normal colorectal tissues. In colerectal carcinomas, the expression rate of Cyclin D1 was 42.69% in uncleus, P16 was 57.30% in cytoplasm and 47.19% in uncleus, CDK4 was 92.13% in cytoplasm, and 22.72% in uncleus, The expression rate of P16 and CDK4 was 25.00% and 41.66% in normal colorectal tissues respectively, and significantly lower than that in colerectal carcinoma (P0.05). The expression of Cyclin D1 was found a significant association with lymph node metastasis and survival rates (P0.05), but no relationship with clinical stage, pathological grade (P0.05). The expression of CDK4 in nucleus was correlated with pathological grade (P0.05), but no relationship with clinical stage, lymph node metastases and survival rate (P0.05). The expression of CDK4 and P16 in cytoplasm and P16 in nucleus was not shown significant association with clinical stage, pathological grade, lymph node metastases and survival rate. Furthermore, significant associations was found between Cyclin D1, P16 and CDK4 expression in nucleus. In addition, p16 gene deletion rate was 47.50%. It has close relation with histological classification (P0.05). There was a positive correlation in expression of p16 gene with P16 protin (P0.05). Conclusions Cyclin D1 is a prognostic indicating tool in colerectal carcinoma, especially in stage Ⅱ. In some extent, the p16 gene detection and CDK4 nuclear expressions are closely associated with cell differentiation. The abnormal regulation of Cyclin D1/CDK4/P16 factors may be one of the causes of colorectal carcinoma.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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