Title: RP-HPLC Determination of Oleanohc Acid in Chaoan capsules
Abstract: Objective To establish an RP-HPLC method for the determination of oleanolic acid in Chaoan capsules Methods A Kromasil-C18 Column(4.6mm×150mm,5um)was used with the mobile phase being aceto-nitrile-water- phosphate(80:20:0.5),flow rate being 0.8 ml.min-1,determinating wavelength being 208 nm,the column temperature being 25℃,Results The calibration curve was linear in the range of 1.124~5.620μg for oleanolic acid(r=0.9999),and the regression equation being Y=381943×X+1651,The average recovery was 100.79%,with RSD of 1.10%.Gonclusion The method is simple,accurate and reproducible for quality control of Chaoan capsules.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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