Title: Inhibitive effect of larcidipine onto systole and diastole of cardiac myocytes
Abstract:Objective To investigate the inhibitive effect and the mechanism of larcidipine on the accentuated myocardial function of systole and diastole enhanced by isoproterenol(Iso),forskolin and MB.Methods T...Objective To investigate the inhibitive effect and the mechanism of larcidipine on the accentuated myocardial function of systole and diastole enhanced by isoproterenol(Iso),forskolin and MB.Methods The systolic amplitude was measured with a video edge tracker method.The following parameters were recorded by computer:ph(peak height),peak height/baseline percent(ph/bl),maximal velocity of systole(+dL/dt)and maximal velocity of diastole(-dL/dt).Results Iso,forskolin and MB increased electrically-induced systole including ph,ph/bl,+dL/dt and-dL/dt.Iso,forskolin and MB augmented the myocardial function of systole and diastole in a significant sense.Iso in a concentration of 10.0nmol/L augmented electrically-induced systole and ph,ph/bl,+dL/dt and-dL/dt,and these parameters increased by 40%,41%,60% and 50%,respectively.Compared with the control group,forskolin in a concentration of 10.0pmol/L increased ph from(0.12±0.03μm to 0.25±0.06μm,ph/bl from 12%±3% to 28%±6%,+dL/dt from 1.8±0.5μm/s to 3.7±0.8μm/s,-dL/dt from 1.8±0.2μm/s to 3.6±0.6μm/s,and these indexes increased by 89%,120%,106% and 86%,respectively.MB in a concentration of 10μmol/L also significantly increased the ph,ph/bl,+dL/dt and-dL/dt to 0.15±0.03μm,12%±4%,2.3±0.4μm/s and 2.4±0.5μm/s,respectively.Larcidipine in a concentration of 3.0μmol/L significantly decreased the parameters of +dL/dt and-dL/dt,which were increased by Iso,forskolin and MB.Conclusions Iso,forskolin and MB may increase the myocardial function of systole and diastole.These drugs might enrich the intracellular calcium indirectly and directly.The effects of Iso,forskolin and MB might be attenuated by Larcidipine.Larcidipine may not only block the dihydropyridine receptor,but also inhibit the other calcium influx.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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