Title: Differentiation of Human Periodontal Ligament Cell Populations to Cementoblasts under Induction of Emdogain:A Preliminary Study
Abstract: Objective To understand the changes in biological characteristics of hPDLPs under induction of EMD.Methods Cultured human periodontal ligament cell populations(hPDLPs) were exposed to the conditioned culture media containing 100 mg/L Emdogain(EMD) for six days.Cell morphology was observed under light microscope,and cell morphology in the cementum chip was observed by in scanning electron microscope before and after induction.Expression of bone sialoprotein,type Ⅰ collagen protein was detected immunohistochemically.Results After induction,cell morphology of hPDLPs changed from a long fusiform to polygon,like cementoblasts rather than the fibroblasts.Immunohistochemistry showed that expression of bone sialoprotein,type Ⅰ collagen was significantly increased.Conclusion EMD could induce differentiation of hPDLPs to cementoblasts.It provide the basis for further research into the regeneration of periodontal tissues and cementum generation.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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