Title: Molecular Cloning and Expression Analysis of Catalase(PuCAT)Gene in Puccinellia chinampoensis
Abstract: The full length cDNA sequence of CATgene was cloned fromPuccinellia chinampoensis leaves using RT-PCR method,the primers were designed according to the homologous CATgene sequences of other plant species.The results showed that the nucleotide sequence of the gene was 1 487 bp,containing a complete open reading frame and encoding 492 amino acids,Genebank:HM230827.Nucleotide and amino acid sequence analysis revealed that PuCAT shared high identity with the orthologs from Triticum aestivum and Hordeum vulgare.And its signal peptide,hydrophobicity/hydrophilic,trans-membrane domain,secondary structure and main functional domains were predicted.Semi-quantitative RT-PCR analysis showed that PuCATexpressed in different tissues,but the expression in root was lower,and in leaf much higher.Various elevated levels of PuCAT expression had been detected when exposed to 4different stress experimental treatments,and the results were not the same.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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